Oogenesis

Oogenesis is the production of secondary oocytes from the primordial germ cells. It takes place in the ovaries.

As a foetus early in development, a female has around 6 million eggs in her ovaries. This number is steadily reduced throughout gestation, so it’s already down to 1-2 million when the baby girl is born. This decrease in egg number continues through childhood, so by the time that baby girl reaches puberty, she has between 300,000 and 400,000 eggs. Before puberty, more than 10,000 eggs die each month. After starting her menstrual cycle, a woman loses about 1,000 every month.

  1. Germinal epithelium cells form oogonia. These are produced before birth.
  2. Oogonia undergo a period of growth and maturation, dividing via mitosis to produce primary oocytes.
  3. The primary oocytes undergo meiosis but the process is halted at prophase 1. At the same time, the germinal epithelium also produces follicle cells that surround the primary oocytes. These are primary follicles.
  4. During puberty, hormones stimulate one of the primary follicles. This matures and becomes a Graafian follicle.
  5. The primary oocytes do not mature until just before ovulation. The primary oocyte completes the first meiotic division to form the haploid secondary oocyte and a small polar body. This polar body can sometimes go on to divide again in meiosis II, forming two polar bodies.
  6. The mature Graafian follicle moves to the surface of the ovary where it releases the secondary oocyte. This is ovulation.
  7. The secondary oocyte begins the second meiotic division, but stops at metaphase 2 unless fertilisation takes place. (Read here that women aren’t going to waste energy on something that may not be fertilised and just lost in menstrual fluid).
  8. Then, as one of my students so aptly put it: it’s like one of those choose your own adventure books: Option a) If fertilisation takes place, meiosis completes and a large ovum and a second polar body is formed. Option b) Fertilisation doesn’t take place and the secondary oocyte is lost with the endometrium during the woman’s period.
  9. After ovulation, the Graafian follicle becomes the corpus luteum (Latin: yellow body). This produces hormones throughout pregnancy that prevents further ovulation and maintains the endometrium. It degenerates if pregnancy does not occur, becoming the corpus albicans (Latin: whitening body), or a scar on the ovary surface that is a remnant of ovulation.

What is the point of the polar bodies?

Polar bodies serve to eliminate one half of the diploid chromosome set produced by meiotic division in the ovum, leaving behind a haploid cell. They have no biological role in fertilisation or embryo development, but by being far smaller in size to the gametocyte that will become the ovum, it ensures that as much cytoplasm as possible will end up in the final ovum.

Describe the process of oogenesis.

●Before birth, oogonia (2n) divide by mitosis to form primary oocytes (2n)

● The primary oocytes begin meiosis I but pause at prophase I until puberty

● During and after puberty, the primary oocyte continues meiosis I, producing a secondary oocyte (n) and a polar body. The secondary oocyte begins to undergo meiosis II, but stops at metaphase II

● If the secondary oocyte is fertilised by a sperm cell, meiosis II finishes and the fertilised egg now has the diploid number of chromosomes.

Important: You are expected to be able to compare and contrast the processes of spermatogenesis and oogenesis.

Compare spermatogenesis and oogenesis.

Similarities:

•Both processes involve the formation of haploid cells by meiosis.

Differences:

•In spermatogenesis, 4 spermatocytes are produced whilst in oogenesis, 1 oocyte and 3 polar bodies are produced.

•There will be more spermatocytes produced compared to oocytes

•In spermatogenesis, spermatocytes are continuously produced whilst in oogenesis, oocytes are generated before birth.

•Spermatocytes are produced throughout a male lifespan whilst oocyte production ceases when there are no more oocytes/at menopause.

•In spermatogenesis, small, motile spermatocytes are produced whilst in oogenesis a large immotile oocyte is produced.

Significance:

•Oocytes and spermatocytes will by haploid, which allows the diploid number to be restored at fertilisation.