DNA structure and replication

DNA Replication

Base pairing allows each strand to serve as a template for a new strand. The new strand is half parent template and half new DNA. This is known as semi-conservative replication.

Stage 1: Unwind DNA

Done by a helicase enzyme

Breaks the hydrogen bonds between the bases

This unwinds part of DNA helix, allowing the separation of the strands.

Stage 2: Build daughter DNA strand

Add new complementary bases.

Done by the enzyme, DNA polymerase.

The enzyme moves 3′ to 5′ and can only add nucleotides to the 3′ end of a growing DNA strand.

Therefore the strand will grow 5′ → 3′.